Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
|
Add ethidium-calcein mixture, and incubate for 30-45 min at RT.
A shorter incubation time may be used if the dye concentrations or incubation temperature are increased. |
|
Protocol tips |
Add ethidium-calcein mixture, and incubate for 30-45 min at RT.
A shorter incubation time may be used if the dye concentrations or incubation temperature are increased. |
Publication protocol
Neonatal cardiomyocytes, ESCs, and noncardiomyocytes including MEFs, HepG2, HEK293, peripheral lymphatic cells, C2C12, skeletal myotubes, and fetal neurons were exposed to glucose-free DMEM (Invitrogen) supplemented with or without lactate (Wako). Cell viabilities were determined by the LIVE/DEAD Viability/Cytotoxicity Assay Kit (Invitrogen) based on the simultaneous determination of live and dead cells with the calcein AM and ethidium homodimer-1 probes. Fluorescence imaging of the cells (live cells were labeled green, whereas the nuclei of dead cells were labeled red) was performed with fluorescence microscopy (IX70 microscope; Olympus) equipped with a color charge-coupled device camera (CS220; Olympus). The green-labeled live area was measured using Image J. Relative cell viabilities were calculated in percentages, compared with those before treatment.
Full paper
Login or
join for free to view the full paper.
Reviews
LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells from Thermo Fisher Scientific has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay mammalian cells - C2C12 using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells from Thermo Fisher Scientific.
Paper title
Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes.
Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells below.
Download manufacturer protocol
Videos
Check out videos that might be relevant for performing Live / Dead assay mammalian cells - C2C12 using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.