CytoTox 96® Non-Radioactive Cytotoxicity Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add reconstituted Substrate Mix to each well of the plate. Incubate at room temperature, protected from light, for 30 minutes

Protocol tips
Add reconstituted Substrate Mix to each well of the plate. Incubate at room temperature, protected from light, for 30 minutes

Publication protocol

Cell viability was assessed using the CytoTox 96 kit (Promega, Madison, WI), which measures the proportion of cellular lactate dehydrogenase (LDH) released into the supernatant.

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Papers

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Manufacturer protocol

Download the product protocol from Promega for CytoTox 96® Non-Radioactive Cytotoxicity Assay below.

Download manufacturer protocol

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