TransIT-TKO Transfection Reagent

siRNA / RNAi /miRNA transfection Human Cells - Primary splenocytes Polymer / lipid

Experiment

siRNA / RNAi /miRNA transfection Human Cells - Primary splenocytes Polymer / lipid

Product

TransIT-TKO Transfection Reagent from Mirus

Manufacturer

Mirus

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-50–100 nM final concentration Mix siRNA:reagent mixture and incubate at room temperature for 15–30 minutes. Add mixture to cells and incubate for 24hours.

Protocol tips
siRNA concentration-50–100 nM final concentration Mix siRNA:reagent mixture and incubate at room temperature for 15–30 minutes. Add mixture to cells and incubate for 24hours.

Publication protocol

siAPC-C (gacgttgcgagaagttgga), siAPC729 (gaggtcatctcagaacaag), siAPCL413 (ggtgtttcctgctgaatga), siAPCL5100 (ggcgccaattcaattgtca), siHIF1α [46], siHIF2α [46] or siGFP (aagctacctgttccatggcca) (50–100 nM final concentration) were transfected into cells overnight using 1 µl Transit TKO (MoBiTec, Göttingen, Germany) per µl siRNA (20 µM). The sequences show only the coding strand.

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Discussion

5 years ago

Author: Keith L. Morrison Canada

siRNA/RNAi/miRNA transfection human

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

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Papers

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Manufacturer protocol

Download the product protocol from Mirus for TransIT-TKO Transfection Reagent below.

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Videos

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