LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 2 µM calcein AM and 4 µM EthD-1. Incubate the cells for 30–45 minutes at room temperature

Protocol tips
Add 2 µM calcein AM and 4 µM EthD-1. Incubate the cells for 30–45 minutes at room temperature

Publication protocol

In order to assess the viability of EPCs embedded into Fibrin-derived gels, EPCs were embedded into Fibrin-derived gels as described above. After gel preparation the LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen) was used to determine the percentage of viable EPCs, according to manufacturer instructions. Samples were visualized on a DF5000B Leica fluorescent microscope and analyzed using LASAF version 2.0.2 software (Leica).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells below.

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