LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Protocol tips

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	Add 4 μM EthD-1 and 2 μM Calcein in PBS) and incubate at 37°C for 30 min

Protocol tips
Add 4 μM EthD-1 and 2 μM Calcein in PBS) and incubate at 37°C for 30 min

Publication protocol

Cell viability was assessed using a Live/Dead Viability/Cytotoxicity Kit [L-3224 Invitrogen, Grand Island, NY]. Briefly, post-treatment, primary hepatocytes were washed with PBS and incubated at 37°C for 30 min with assay reagent (4 μM EthD-1 and 2 μM Calcein in PBS) at 37°C. The cells were removed and washed three times with PBS and viewed with an Axiovert 40 CFL [Zeiss, Germany] and X-Cite 120Q [Lumin Dynamics, Mississauga, Ontario, Canada].

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells below.

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