Live and Dead Cell Assay (Abcam)

Protocol tips

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	Add 1X Live and Dead Dye to cells Incubate 10 min at room temperature

Protocol tips
Add 1X Live and Dead Dye to cells Incubate 10 min at room temperature

Publication protocol

1 × 106 of each type of MSC pellet was diluted with 100 μl of HBSS (MSC/HBSS suspension), and subsequently resuspended with 100 μl of PM mixture (0.2–2.5%) containing sucrose solution with corresponding concentration. Same number of cells was suspended with 200 μl of PBS as a control (without PM). 5 μl of propidium iodide (PI) solution (Calbiochem, UK) was added to the PM-MSC complex. After 10 and 60 min observation, 10 μl of that was taken and placed on the slide (Thermo Scientific, UK), then enclosed with round type cover glass (VWR, UK). PI-positive cells were detected and analyzed by fluorescence microscopy (BZ8000; Keyence). In addition, cell viability was confirmed by using a Live and Dead Cell Assay kit (Abcam, UK) according to the company's instruction.

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