RealTime-Glo™ MT Cell Viability Assay

Protocol tips

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	Add 16 uL of CellTiter-Blue dye to wells containing 80 μL of cell culture media and incubate cells in a cell culture incubator at 37°C and 5% CO2 for 1 hour

Protocol tips
Add 16 uL of CellTiter-Blue dye to wells containing 80 μL of cell culture media and incubate cells in a cell culture incubator at 37°C and 5% CO2 for 1 hour

Publication protocol

All assays purchased from Promega were performed according to the instructions of the manufacturer using a SpectraMax M5 multi-mode microplate reader (Molecular Devices, Sunnyvale, California). CellTiter-Blue assays (cat #G8081, Promega) were performed by the addition of 16 uL of CellTiter-Blue dye to wells containing 80 μL of cell culture media and measured by the increase in fluorescence (560 nm excitation, 590 nm emission). The ApoTox-Glo Triplex Assay (cat #G6320) was used to assess viability, toxicity, and caspase 3/7 enzymatic activity. The RealTime-Glo MT Viability Assay was used to assess viability in real time over a time course of 24 h. The GSH-Glo Glutathione Assay was used to quantify reduced glutathione.

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Manufacturer protocol

Download the product protocol from Promega for RealTime-Glo™ MT Cell Viability Assay below.

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