Live and Dead Cell Assay (Abcam)

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	Incubate cells for 15 min at 37 °C

Protocol tips
Incubate cells for 15 min at 37 °C

Publication protocol

Cell viability was determined with a live/dead assay kit (Abcam, Cambridge, UK). To perform viability assays in cardiomyocytes that were cultured with DMEM containing 1 wt% GC or 1 wt% HGC, 12 mm collagen-coated cover slips were placed in the wells of a 24-well tissue culture plate, and cells were seeded in these plates at a density of 1 × 105 per well in DMEM. After 1 day of incubation at 37 °C, the medium was exchanged with DMEM containing 1 wt % GC or 1 wt% HGC. On day 3, the media were replaced with a staining solution, and the samples were then incubated for 15 min at 37 °C and examined via fluorescence microscopy. The viability of aggregates on GC- or HGC-coated dishes was also measured by live/dead assay and observed using laser scanning confocal microscopy (LSM 710, Carl Zeiss, Thornwood, NY, USA)

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