CellTiter-Glo® Luminescent Cell Viability Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add reagent and mix contents for 2 minutes to induce cell lysis. Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal.

Protocol tips
Add reagent and mix contents for 2 minutes to induce cell lysis. Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal.

Publication protocol

Cell viability was measured using the MTS-based Cell Titer 96®AQueous One solution cell proliferation assay (Promega, Madison, WI) or CellTiter-Glo® luminescent cell viability assay (Promega) according to the manufacturer's instructions. Upon addition of MTS solution, the reaction plate was incubated at 37°C for 3 h, and the absorbance was read at 490 nm with a plate reader (TECAN, Männedorf, Switzerland). For phosphatidylserine exposure, cells were stained with annexin V-PE as described by the manufacturer (BD Biosciences, San Jose, CA), and assayed by flow cytometry (CyAn ADP, Beckman Coulter, Brea, CA, USA)

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay mammalian cells - GH3 using CellTiter-Glo® Luminescent Cell Viability Assay from Promega.

Manufacturer protocol

Download the product protocol from Promega for CellTiter-Glo® Luminescent Cell Viability Assay below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Live / Dead assay mammalian cells - GH3 using CellTiter-Glo® Luminescent Cell Viability Assay from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.