Cell Counting Kit-8

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Pre-incubate the plate in a humidified incubator (e.g., at 37°C, 5% CO2)	Add CCK-8 solution and incubate the plate for 4 hours in the incubator	. Measure and subtract the O.D. at 600 nm or higher from that of sample if there is a high turbidity in the cell suspension

Upstream tips
Pre-incubate the plate in a humidified incubator (e.g., at 37°C, 5% CO2)
Protocol tips
Add CCK-8 solution and incubate the plate for 4 hours in the incubator
Downstream tips
. Measure and subtract the O.D. at 600 nm or higher from that of sample if there is a high turbidity in the cell suspension

Publication protocol

Cell survival was quantified using Cell Counting Kit-8 (Dojindo Laboratories, Tokyo, Japan). In brief, GH3 cells were cultured in 96-well plates (Corning Inc., Corning, NY, USA) at a density of 5×103 cells per well. The cells were cultured in the presence or absence of melatonin. After 15 h, cells were washed, treated with Cell Counting Kit-8 reagents, incubated in the dark for 4 h, and then absorbance (450 nm) was measured using a plate reader (Molecular Device, Sunnyvale, CA). Percent viability was calculated as the absorbance of the melatonin-treated sample/control absorbance ×100.

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Manufacturer protocol

Download the product protocol from Dojindo for Cell Counting Kit-8 below.

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