CellTiter-Glo® Luminescent Cell Viability Assay

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	Allow the plate to incubate at 37C for 3h minutes to stabilize luminescent signal

Protocol tips
Allow the plate to incubate at 37C for 3h minutes to stabilize luminescent signal

Publication protocol

Cell viability was measured using the MTS-based Cell Titer 96®AQueous One solution cell proliferation assay (Promega, Madison, WI) or CellTiter-Glo® luminescent cell viability assay (Promega) according to the manufacturer's instructions. Upon addition of MTS solution, the reaction plate was incubated at 37°C for 3 h, and the absorbance was read at 490 nm with a plate reader (TECAN, Männedorf, Switzerland). For phosphatidylserine exposure, cells were stained with annexin V-PE as described by the manufacturer (BD Biosciences, San Jose, CA), and assayed by flow cytometry (CyAn ADP, Beckman Coulter, Brea, CA, USA)

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Manufacturer protocol

Download the product protocol from Promega for CellTiter-Glo® Luminescent Cell Viability Assay below.

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