Cell Counting Kit-8 (CCK-8)

Protocol tips

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	Add 10ul of reagent and incubate the plate for 1 hour in the incubator.

Protocol tips
Add 10ul of reagent and incubate the plate for 1 hour in the incubator.

Publication protocol

The effect of TNF-α on cell viability was carried out using Cell Counting Kit-8 (Boster Co.). Briefly, MMQ cells were plated in 96-well plates (20,000/well). The cells were treated with TNF-α at concentrations of 0, 10, 20 ng/mL. After 24 h incubation, 10 μL of the CCK-8 solution was then added to each well of the plate, and then the plate was incubated for another 1 h. The absorbance was measured at 450 nm using a 96-well plate reader.

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Manufacturer protocol

Download the product protocol from BosterBio for Cell Counting Kit-8 (CCK-8) below.

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