CellTiter-Glo® Luminescent Cell Viability Assay

Protocol tips

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	Add test compounds and equilibrate the plate and its contents at room temperature for approximately 15 minutes. Add CellTiter-Glo® Reagent and mix contents for 2 minutes on an orbital shaker to induce cell lysis. Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal

Protocol tips
Add test compounds and equilibrate the plate and its contents at room temperature for approximately 15 minutes. Add CellTiter-Glo® Reagent and mix contents for 2 minutes on an orbital shaker to induce cell lysis. Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal

Publication protocol

Cellular viability was measured using the CellTiter-Glo® Luminescent cell viability assay. Briefly, CellTiter-Glo (Promega) buffer was thawed and equilibrated to room temperature. The lyophilized powder CellTiter-Glo substrate was allowed to equilibrate to room temperature. The CellTiter-Glo Buffer in the appropriate volume (10 mL in pack A, 100 mL in pack B) was transferred into a brown bottle containing the CellTiter-Glo substrate to prepare the enzyme/substrate mixture, which formed the CellTiter-Glo reagents. The H9C2 cardiomyocytes were plated in 96-well plates. After 16 h, the cells were divided into 8 groups and treated with different drugs. After 24 h incubation at 37°C, the plates were incubated at room temperature for 15 minutes. Then, 100 μL of CellTiter-Glo reagent were added to each well and the contents were mixed on an orbital shaker for 2 minutes to induce cell lysis. The 96-well plates were incubated at room temperature for 10 minutes, so that the value of fluorescence signal stabilizes. A microplate reader was used to detect the fluorescence signal.

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Manufacturer protocol

Download the product protocol from Promega for CellTiter-Glo® Luminescent Cell Viability Assay below.

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