|Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature.
Incubate cells for 18h at 37°C and change medium and re-incubate for 72 hours
Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
2 years ago
Author: Keith L. Morrison
I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?
Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / RNAi /miRNA transfection Human Cells - HESC Lipofectamine using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific.
Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.Download manufacturer protocol
Check out videos that might be relevant for performing siRNA / RNAi /miRNA transfection Human Cells - HESC Lipofectamine using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Fill out your contact details and receive price quotes in your InboxOutsource experiment