Live/Dead cell Staining Kit II

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 100 µl of the Calcein-AM/EthD-III to each well. Incubate the samples at room temperature for 30–45 minutes

Protocol tips
Add 100 µl of the Calcein-AM/EthD-III to each well. Incubate the samples at room temperature for 30–45 minutes

Publication protocol

Living and dead cell number was assayed using the Live and Dead Assay from Promokine (PK-CA707–30002) as suggested by the manufacturer. A calibration curve was realized by seeding incremental number of cells and counting viable cells after 96 h. For the dead cell calibration, 100% dead cells were obtained after 30 min ethanol treatment. For the experiment, cells were seeded at a density of 6000 cells/well in 96-well plates in quintuplicate. Cells were transfected 24 h after plating and total live and dead cell number was quantified 72 h after transfection (96h total) on a TECAN Infinite F500 plate reader at 515 nm (green-fluorescent Calcein AM) for live cells and 620 nm (red-fluorescent ethidium homodimer III) excited respectively at 490 and 530 nm.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay mammalian cells - H9C2 using Live/Dead cell Staining Kit II from PromoKine.

Manufacturer protocol

Download the product protocol from PromoKine for Live/Dead cell Staining Kit II below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Live / Dead assay mammalian cells - H9C2 using Live/Dead cell Staining Kit II from PromoKine. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.