Cell Counting Kit-8

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 10 μl CCK-8 solution incubate for 2 h.	Absorbance of each wells was measured at 450 nm To measure the absorbance later, add 10 μl of 1% w/v SDS or 0.1 M HCl to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 24 hours

Protocol tips
Add 10 μl CCK-8 solution incubate for 2 h.
Downstream tips
Absorbance of each wells was measured at 450 nm To measure the absorbance later, add 10 μl of 1% w/v SDS or 0.1 M HCl to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 24 hours

Publication protocol

Cell viability was measured using Cell Counting Kit-8 (CCK-8; Dojindo, Japan). NP cells were plated in 96-well plates at a density of 5 × 103 per well, incubate with various concentrations of piperine for 24 h, then add 10 μl CCK-8 solution incubate for 2 h. Absorbance of each wells was measured at 450 nm. The culture medium was used as a blank. Cell viability was calculated as: cell viability = [absorbance (with piperine)-absorbance (blank)]/[absorbance (without piperine)-absorbance (blank)].

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay mammalian cells - rat nucleus pulposus using Cell Counting Kit-8 from Dojindo.

Manufacturer protocol

Download the product protocol from Dojindo for Cell Counting Kit-8 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Live / Dead assay mammalian cells - rat nucleus pulposus using Cell Counting Kit-8 from Dojindo. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.