LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy & quantitative assays

Protocol tips

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	Add 0.5 mL of dye solution containing 1 mM SYTO9 and 5 mM propidium iodide (PI). Incubate for 20 min at room temperature.

Protocol tips
Add 0.5 mL of dye solution containing 1 mM SYTO9 and 5 mM propidium iodide (PI). Incubate for 20 min at room temperature.

Publication protocol

Live/dead bacterial assay was carried out to examine the effect of nanoparticles on the integrity of bacterial cell membranes.41 10 mg mL
1 Au@Ag NPs were added to 107 CFU mL 1 bacterial cells, the mixture was incubated at 37 C for 24 h. Then the solutions were mixed with 0.5 mL of dye solution containing 1 mM SYTO9 and 5 mM propidium iodide (PI) for 20 min at room temperature. The bacteria cells were imaged using a confocal laser scanning microscope (TCS-SP2, Leica, Germany).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy & quantitative assays below.

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