LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy

Protocol tips

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	Add 3 µL of the dye mixture for each mL of the bacterial suspension. Mix thoroughly and incubate at room temperature in the dark for 15 minutes

Protocol tips
Add 3 µL of the dye mixture for each mL of the bacterial suspension. Mix thoroughly and incubate at room temperature in the dark for 15 minutes

Publication protocol

Biofilm development in the presence of dead cells at different concentrations was monitored with sterile, non-treated 96-well flat-bottom microtiter plates. The purpose of these experiments was to better interpret the results of biofilm formation in the presence of different minerals. Specifically, these experiments attempted to test the hypothesis that dead cells can serve as a nutrient source to enhance biofilm development. The cells were inactivated by autoclave treatment. To ensure that all the cells were dead, a sterility test was performed in triplicate by plating 50 µL of autoclaved cells on LB plates. A serial dilution (10, 10, 10, 10) of the 3 × 10 CFU mL dead cells was performed in MSgg to obtain different concentrations of dead cells for the biofilm assay. The biofilm growth procedure was the same as described above, except that instead of MSgg different concentrations of dead cells were used. The biofilms were measured after 48 h of growth at 28 °C using the same procedures described in the section “Assays of biofilm development in suspended minerals”.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy below.

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