LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy

Live / Dead assay bacteria - Porphyromonas gingivalis

Experiment

Live / Dead assay bacteria - Porphyromonas gingivalis

Product

LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 3 µL of the dye mixture for each mL of the bacterial suspension. Mix thoroughly and incubate at room temperature in the dark for 15 minutes.

Protocol tips
Add 3 µL of the dye mixture for each mL of the bacterial suspension. Mix thoroughly and incubate at room temperature in the dark for 15 minutes.

Publication protocol

The biofilm from the MRD was stained using a Live/Dead® BacLight™ Bacterial Viability Kit (L7007; Molecular Probes, Eugene, OR) and observed by confocal laser scanning microscopy (LSM-700; Carl Zeiss, München-Hallbergmoos, Germany) as described previously55.

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Discussion

5 years ago

Author: M. Daecher Germany

Live/dead assay Bacteria

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay bacteria - Porphyromonas gingivalis using LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy from Thermo Fisher Scientific.

Paper title

Aggravation of collagen-induced arthritis by orally administered through modulation of the gut microbiota and gut immune system

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy below.

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