Cytotoxicity Detection Kit (LDH)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate the cells in an incubator (37°C, 5% CO2, 90% humidity

Protocol tips
Incubate the cells in an incubator (37°C, 5% CO2, 90% humidity

Publication protocol

Percent cytotoxicity for each corneal isolate was determined by measuring the amount of lactate dehydrogenase released into the media by dead or dying host cells after bacterial exposure using a cytotoxicity detection kit (Roche Diagnostics, Indianapolis, IN). Values were compared to a positive control, P. aeruginosa clinical isolate 6206. In some experiments, invasive control 6294 had slightly lower LDH values than the media (baseline) control. Negative values for percent cytotoxicity corresponded to strains that were not cytotoxic and had negative LDH values after the media control had been included in the calculation. Values greater than one corresponded to invasiveness or cytotoxicity greater than the respective positive control.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay bacteria - Pseudomonas aeruginosa using Cytotoxicity Detection Kit (LDH) from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Cytotoxicity Detection Kit (LDH) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Live / Dead assay bacteria - Pseudomonas aeruginosa using Cytotoxicity Detection Kit (LDH) from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.