LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Protocol tips

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	Stain cells with dyes to a final concentration of 3.34 and 20 μM (SYTO 9 and PI, respectively). Incubate at room temperature in the dark for 15 min

Protocol tips
Stain cells with dyes to a final concentration of 3.34 and 20 μM (SYTO 9 and PI, respectively). Incubate at room temperature in the dark for 15 min

Publication protocol

Live/Dead assay kit was used both on C. albicans biofilms and plancktonic cells. LIVE/DEAD® FungaLight™ Yeast Viability Kit (L34952, LifeTechnologies, USA) is composed by two fluorescent probes, SYTO 9 and propidium iodide (PI). In a population of live and dead cells, SYTO 9 nucleic acid labels all yeast in a population, those with intact membranes and those with damaged membranes. In contrast, PI penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present (Johnson and Spence, 2010). As a result, yeast with intact membranes is stained with green fluorescence, whereas yeast with damaged membranes is stained with red fluorescence.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry below.

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