BacTiter-Glo™ Microbial Cell Viability Assay

Protocol tips

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	Add a volume of BacTiter-Glo™ Reagent equal to the volume of cell culture medium present in each well. Mix contents briefly on an orbital shaker and incubate for five minutes

Protocol tips
Add a volume of BacTiter-Glo™ Reagent equal to the volume of cell culture medium present in each well. Mix contents briefly on an orbital shaker and incubate for five minutes

Publication protocol

Determination of the number of viable yeast cells was based on quantitation of ATP; cells were assessed with BactTiter-Glo™ Microbial Cell Viability Assay according to the manufacturer's protocol (Promega). Cells were suspended in a 100 mM phosphate buffer with pH 7.0, containing 0.1% glucose and 1 mM EDTA. A sample (100 μL) of cell suspension with density 106 cells mL−1 was used for determination purposes. Luminescence was recorded after 5 min using TECAN Infinite 200 microplate reader. The luminescent signal was proportional to the amount of ATP present, which was directly proportional to the number of cells.

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Manufacturer protocol

Download the product protocol from Promega for BacTiter-Glo™ Microbial Cell Viability Assay below.

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