LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Protocol tips

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	Add 1 µL of SYTO®9 dye and 1 µL of propidium iodide. Incubate at room temperature or 37ºC protected from light for 15–30 minutes

Protocol tips
Add 1 µL of SYTO®9 dye and 1 µL of propidium iodide. Incubate at room temperature or 37ºC protected from light for 15–30 minutes

Publication protocol

LIVE/DEAD FungaLight Yeast Viability Kit (Life Technologies) was used in compliance with manufacturer instructions to access fraction of dead yeast cells and/or cells with compromised membranes in five strains of S90 background (Δure2, Δgtr1, Δdal81, Δgln3 and the wild type). Exponentially growing cells were washed and re-suspended in Tris buffered saline solution, pH 7 to produce a suspension of 0.4 OD600. Cells were stained with PI and SYTO® 9 fluorescent dyes and counted with LSR-Fortessa analyzer (Beckton Dickinson). Fluorescent events were recorded at excitation/emission 480/500 nm for SYTO9 and 490/635 nm for PI. Gating was adjusted to exclude debris and cell duplicates. Each sample was measured until the minimum of 2000 of dead/damaged cells was reached. See Figures S7B and S7C for the results.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry below.

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