Live and Dead Cell Assay (Abcam)

Protocol tips

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	Incubate cells for 30min at room temperature.

Protocol tips
Incubate cells for 30min at room temperature.

Publication protocol

For analysis of cell viability, the thermogelling cell suspension was allowed to gel at 37°C in a humidified atmosphere of 5% CO2 for 10 min before the addition of culture medium (DMEM, 10% FBS, 1% P/S) to the formed gels. The medium was changed every 3 days. The viability of the encapsulated BMSCs was assessed using a Live/Dead assay kit (Abcam, Sigma-Aldrich). The cell-gel constructs were incubated in a standard working solution at room temperature for 30 min and then observed under a confocal laser scanning microscope (Nikon A1R, Japan). The nuclei of live cells stained bright green, and dead cells stained red. Additionally, cell viability was also assessed by the MTT assay as previously described [29].

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