EZ DNA Methylation kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Bisulfite Treatment and DNA Purification
Genomic DNA was chemically modified using sodium bisulfite, which converts all unmethylated cytosine to uracil but leaves methylated cytosine unmodified. To this end, we used an EZ DNA methylation kit (Zymo Research, Irvine, CA) according to the manufacturer's instructions. In brief, genomic DNA was treated with sodium bisulfite for 2.5 hours at 65°C and desulfonation was performed for 20 minutes at room temperature. Bisulfite-converted DNA was purified using a Zymo-Spin IC column (Zymo Research) and eluted with 10 μL of distilled water. The eluted DNA was either used immediately for methylation analysis or was stored at −20°C until further use.

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - Caco-1 CHST11 using EZ DNA Methylation kit from Zymo Research.

Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Gene specific profiling - Caco-1 CHST11 using EZ DNA Methylation kit from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.