CpGenome Universal DNA Modification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Bisulfite sequencing PCR (BSP)
Genomic DNA was isolated by use of a DNeasy Blood & Tissue kit. Isolated DNA was subjected to modification by use of a CpGenome DNA modification kit according to the manufacturer's recommendations and amplified via PCR with primers for the ERα promoter region. From the bisulfite-modified DNA, the ERα promoter was amplified by PCR under conditions described previously . ERS primers were and . PCR products were gel purified and cloned using the pEASY-T3 Cloning Kit (Beijing TransGen Biotech Co., Ltd.). Ten random clones were sequenced by the Invitrogen Company.



Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - MCF-7 Estrogen receptor alpha using CpGenome Universal DNA Modification Kit from Millipore.

Manufacturer protocol

Download the product protocol from Millipore for CpGenome Universal DNA Modification Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Gene specific profiling - MCF-7 Estrogen receptor alpha using CpGenome Universal DNA Modification Kit from Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.