CpGenome Universal DNA Modification Kit

DNA methylation profiling Gene specific profiling - MCF-7 Estrogen receptor alpha

Experiment
DNA methylation profiling Gene specific profiling - MCF-7 Estrogen receptor alpha
Product
CpGenome Universal DNA Modification Kit from Millipore
Manufacturer
Millipore

Protocol tips

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Bisulfite sequencing PCR (BSP)
Genomic DNA was isolated by use of a DNeasy Blood & Tissue kit. Isolated DNA was subjected to modification by use of a CpGenome DNA modification kit according to the manufacturer's recommendations and amplified via PCR with primers for the ERα promoter region. From the bisulfite-modified DNA, the ERα promoter was amplified by PCR under conditions described previously . ERS primers were and . PCR products were gel purified and cloned using the pEASY-T3 Cloning Kit (Beijing TransGen Biotech Co., Ltd.). Ten random clones were sequenced by the Invitrogen Company.



Full paper   Login or join for free to view the full paper.

Reviews

CpGenome Universal DNA Modification Kit from Millipore has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - MCF-7 Estrogen receptor alpha using CpGenome Universal DNA Modification Kit from Millipore.

Paper title
Arsenic Induces Functional Re-Expression of Estrogen Receptor α by Demethylation of DNA in Estrogen Receptor-Negative Human Breast Cancer
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Millipore for CpGenome Universal DNA Modification Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Gene specific profiling - MCF-7 Estrogen receptor alpha using CpGenome Universal DNA Modification Kit from Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms