EpiTect Methyl II PCR Assays

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Epitect methyl qPCR arrays
To identify relevant gene promoter methylation we used EpiTect Methyl qPCR Arrays (SA Biosciences, Qiagen, Hilden, Germany) as a screening method. We analyzed 96 cancer-related genes from four pathways including stem cell transcription factors, homeobox genes, wnt signaling, and epithelial to mesenchymal transition (EMT). Assays were performed according to the manufacturer’s instructions. Results were displayed as percentage of unmethylated and methylated fraction.



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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - MCF-7 FOXA2 using EpiTect Methyl II PCR Assays from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for EpiTect Methyl II PCR Assays below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Gene specific profiling - MCF-7 FOXA2 using EpiTect Methyl II PCR Assays from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.