CpGenome Universal DNA Modification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Methylation-specific PCR (MSP) and bisulfite sequencing PCR (BSP)
DNA from tissue samples and cell lines were subjected to bisulfite treatment using CpGgenome DNA Modification Kit (Chemicon, USA). Sequences, Tm, and product length of each primer used for MSP and BSP analysis are summarized in Table ​Table11 The band expanded with methylation-specific PCR primers corresponding to the DNA methylation in the promoter region was marked as "M". The band expanded with non-methylation-specific primers was marked as "U".

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - Human ovarian tissue TGFBI using CpGenome Universal DNA Modification Kit from Millipore.

Manufacturer protocol

Download the product protocol from Millipore for CpGenome Universal DNA Modification Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Gene specific profiling - Human ovarian tissue TGFBI using CpGenome Universal DNA Modification Kit from Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.