EZ DNA Methylation kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Methylation specific polymerase chain reaction (MSP): DNA methylation pattern in CpG island of MGMT gene was determined by nested MSP of bisulphite converted DNA. DNA bisulphite conversion was performed using a commercially available kit (EZ DNA methylation kit, Zymo Research, California, USA) following the manufacturer's instructions. DNA was treated with sodium bisulphite, to convert all unmethylated cytosine to uracil, whereas methylated cytosine remained unchanged. Bisulphite converted DNA was stored at -70°C until used.

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - Human ovarian tissue MGMT using EZ DNA Methylation kit from Zymo Research.

Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Gene specific profiling - Human ovarian tissue MGMT using EZ DNA Methylation kit from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.