EZ DNA Methylation kit

DNA methylation profiling Gene specific profiling - Human ovarian tissue MGMT

Experiment
DNA methylation profiling Gene specific profiling - Human ovarian tissue MGMT
Product
EZ DNA Methylation kit from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Methylation specific polymerase chain reaction (MSP): DNA methylation pattern in CpG island of MGMT gene was determined by nested MSP of bisulphite converted DNA. DNA bisulphite conversion was performed using a commercially available kit (EZ DNA methylation kit, Zymo Research, California, USA) following the manufacturer's instructions. DNA was treated with sodium bisulphite, to convert all unmethylated cytosine to uracil, whereas methylated cytosine remained unchanged. Bisulphite converted DNA was stored at -70°C until used.

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Reviews

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Discussion

Discussion

5 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - Human ovarian tissue MGMT using EZ DNA Methylation kit from Zymo Research.

Paper title
Relationship between promoter methylation & tissue expression of gene in ovarian cancer
Full paper
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Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

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Videos

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