MethylMiner™ Methylated DNA Enrichment Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

DNA isolation and MethylCap-seq assay
Genomic DNA was extracted from ovarian tissue or cell lines using the QIAmp DNA Mini Kit (QIAGEN GmbH). Methylated DNA was enriched from 2 µg genomic DNA using the MethylMiner Methylated DNA Enrichment Kit (Invitrogen) following the manufacturer?s instructions. Briefly, genomic DNA was sonicated to ~200-bp, captured by MBD proteins and precipitated using 1M salt buffer. Enriched methylated DNA was used to generate libraries for sequencing following the standard protocols from Illumina. MethylCap-seq libraries were sequenced using the Illumina Genome Analyzer IIx System. Image analysis and base calling were performed using the standard Illumina pipeline. Unique reads (up to 36-bp reads) were mapped to the human reference genome (hg18) using the ELAND algorithm, with up to two base-pair mismatches (see ).

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Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - SKOV3 ZIC1 using MethylMiner™ Methylated DNA Enrichment Kit from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MethylMiner™ Methylated DNA Enrichment Kit below.

Download manufacturer protocol

Videos

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