MethylMiner™ Methylated DNA Enrichment Kit

DNA methylation profiling Gene specific profiling - SKOV3 ZIC1

Experiment
DNA methylation profiling Gene specific profiling - SKOV3 ZIC1
Product
MethylMiner™ Methylated DNA Enrichment Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

DNA isolation and MethylCap-seq assay
Genomic DNA was extracted from ovarian tissue or cell lines using the QIAmp DNA Mini Kit (QIAGEN GmbH). Methylated DNA was enriched from 2 µg genomic DNA using the MethylMiner Methylated DNA Enrichment Kit (Invitrogen) following the manufacturer?s instructions. Briefly, genomic DNA was sonicated to ~200-bp, captured by MBD proteins and precipitated using 1M salt buffer. Enriched methylated DNA was used to generate libraries for sequencing following the standard protocols from Illumina. MethylCap-seq libraries were sequenced using the Illumina Genome Analyzer IIx System. Image analysis and base calling were performed using the standard Illumina pipeline. Unique reads (up to 36-bp reads) were mapped to the human reference genome (hg18) using the ELAND algorithm, with up to two base-pair mismatches (see ).

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Reviews

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Discussion

Discussion

5 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - SKOV3 ZIC1 using MethylMiner™ Methylated DNA Enrichment Kit from Thermo Fisher Scientific.

Paper title
Comprehensive methylome analysis of ovarian tumors reveals hedgehog signaling pathway regulators as prognostic DNA methylation biomarkers
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MethylMiner™ Methylated DNA Enrichment Kit below.

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Videos

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