EZ DNA Methylation-Gold Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Protocol tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Methylation specific PCR (MSP)
DNA was isolated using standard phenol:chloroform extraction and quantified using an ND1000 spectrophotometer (Thermo Scientific). DNA (1 μg) was subjected to bisulfite modification using EZ gold methylation kit (Zymo Research). Bisulfite-modified DNA was used for MSP of the TIMP2 gene with a set of primers (9) spanning regions 1919–1987 (−325 to −257), relative to the transcription start site. MSP was performed as detailed by Ivanova et al (9). The PCR products were analysed on 3% agarose gel. MSP was carried out in duplicate.

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - HeLa TIMP2 using EZ DNA Methylation-Gold Kit from Zymo Research.

Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation-Gold Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Gene specific profiling - HeLa TIMP2 using EZ DNA Methylation-Gold Kit from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.