CpGenome Universal DNA Modification Kit

DNA methylation profiling Gene specific profiling - HeLa P16

Experiment
DNA methylation profiling Gene specific profiling - HeLa P16
Product
CpGenome Universal DNA Modification Kit from Millipore
Manufacturer
Millipore

Protocol tips

Protocol tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

2.6. Bisulfite treatment
Of the DNA extracts, 15 l of the tissue extract, and 50 l each of the plasma and EBC extracts, was used for bisulfite treatment. Bisulfite treatment was performed using the CpGenomeTM DNA Modification Kit (Millipore, Billerica, MA, USA), with the reaction condition optimized to 50 ◦C for 14 h. Finally, DNA was eluted in 30 l of TE.

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Reviews

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Discussion

Discussion

2 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - HeLa P16 using CpGenome Universal DNA Modification Kit from Millipore.

Paper title
Methylation of P16 in exhaled breath condensate for diagnosis of non-small cell lung cancer.
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Manufacturer protocol

Download the product protocol from Millipore for CpGenome Universal DNA Modification Kit below.

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Videos

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