Imprint® Methylated DNA Quantification Kit

DNA methylation profiling Gene specific profiling - MRC-5 LSH

Experiment

DNA methylation profiling Gene specific profiling - MRC-5 LSH

Product

Imprint® Methylated DNA Quantification Kit from Sigma-Aldrich

Manufacturer

Sigma-Aldrich

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Ensure DNA is mixed thoroughly prior to application. The wells of the assay strip bind up to 200 ng of total DNA.

Protocol tips
Ensure DNA is mixed thoroughly prior to application. The wells of the assay strip bind up to 200 ng of total DNA.

Publication protocol

DNA methylation assays
The global levels of 5-methyl cytosine in the genome of MRC5hTERT, LSH KD and control KD cells were investigated by ELISA assays using an ImprintTM kit (Sigma Aldrich) according to manufacturer’s instructions.

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Reviews

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Discussion

5 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - MRC-5 LSH using Imprint® Methylated DNA Quantification Kit from Sigma-Aldrich.

Paper title

The SNF2 family ATPase LSH promotes phosphorylation of H2AX and efficient repair of DNA double-strand breaks in mammalian cells

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Imprint® Methylated DNA Quantification Kit below.

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Videos

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