EpiTect Bisulfite Kit

DNA methylation profiling Gene specific profiling - MRC-5 BARX1

Experiment
DNA methylation profiling Gene specific profiling - MRC-5 BARX1
Product
EpiTect Bisulfite Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

MSP
We used MSP11 to determine the methylation status of the 25 selected regions in 122 samples (61 tumors and matching healthy tissues). Samples were bisulfite converted using the Epitect kit from Qiagen (Germantown, MD) following the manufacturer's protocol. Samples were amplified using two different set of primers designed for the methylated and unmethylated sequences. Primers and amplification conditions are specified in Supplementary Table 1 (Supplemental Digital Content 1, http://links.lww.com/JTO/A394). The fully methylated and fully unmethylated DNA samples were used as controls, and water blank reactions were used as controls for contamination. After amplification, products were resolved on 2% agarose gels containing ethidium bromide.

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Discussion

Discussion

5 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - MRC-5 BARX1 using EpiTect Bisulfite Kit from Qiagen.

Paper title
Genomewide DNA methylation analysis identifies novel methylated genes in non-small-cell lung carcinomas.
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Manufacturer protocol

Download the product protocol from Qiagen for EpiTect Bisulfite Kit below.

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Videos

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