PCR SuperMix

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.	Always thaw on ice

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.
Protocol tips
Always thaw on ice

Publication protocol

Endpoint PCR and qPCR

Endpoint PCR was conducted in a total volume of 20 µL containing 0.4 µM each primer (), 0.25 mM each dNTP, 1 U or 4 U FastStart DNA polymerase (Roche, Basel, Switzerland) and 1 x FastStart PCR reaction buffer with 2 mM MgCl. Cycling conditions were at 95°C for 15 min and 40 cycles at 95°C/30 s, 54°C/30 s, 72°C/30 s.


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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PCR SuperMix below.

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