Polybrene Infection / Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not add antibiotics to the Transfection Medium

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

The SMMC-7721 cells were incubated with the viral stock containing 8 mg/L polybrene. On the following day, the cells were split and selected in 500 mg/L G418. Cultures were added every 3-4 d with the fresh G418 supplemented media for 14 d. Resistant colonies were expanded, and subcloned and the clone which produced the reduced levels of VEGF was selected for further research.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Polybrene Infection / Transfection Reagent below.

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