Upstream tips |
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Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp. |
Protocol tips |
Always thaw on ice |
Hot Start Taq DNA Polymerase from GenScript has not yet been reviewed for this experiment
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Discussion
5 years ago
Author:
I keep getting a non-specific band in PCR so I would like to try a hot start PCR manually. How should I prepare the mix and at which step should I be adding the polymerase?
Discussion
5 years ago
Author: Janina Reinhardt
I am currently performing hot start PCR using phusion green hot start DNA polymerase in order to amplify a 8.8kb insert. However, I cannot get any amplified product at all. I have tried changing some parameters but I am not sure what to do. Any help?
Check out relevant papers found by Labettor's AI that are relevant for performing PCR Hot start PCR - Mammalian DNA using Hot Start Taq DNA Polymerase from GenScript.
Download the product protocol from GenScript for Hot Start Taq DNA Polymerase below.
Download manufacturer protocolCheck out videos that might be relevant for performing PCR Hot start PCR - Mammalian DNA using Hot Start Taq DNA Polymerase from GenScript. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
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