Hot Start Taq DNA Polymerase

PCR Hot start PCR - Mammalian DNA

Experiment
PCR Hot start PCR - Mammalian DNA
Product
Hot Start Taq DNA Polymerase from GenScript
Manufacturer
GenScript

Protocol tips

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.
Protocol tips
Always thaw on ice

Publication protocol

Total RNA was extracted from cerebral cortex of control and SAH rats, and cDNA was synthesized as described above. Amplification of cDNA was performed using Taq DNA polymerase (GenScript) and the primers listed in Table S1. Band intensity in the linear range of amplification was quantified and normalized to band intensity values for GAPDH

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Reviews

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Discussion

Discussion

5 years ago

Author: Qatar

When should I add the polymerase for hot start PCR?

I keep getting a non-specific band in PCR so I would like to try a hot start PCR manually. How should I prepare the mix and at which step should I be adding the polymerase?

Discussion

5 years ago

Author: Janina Reinhardt Switzerland

Hot start PCR using phusion green DNA polymerase

I am currently performing hot start PCR using phusion green hot start DNA polymerase in order to amplify a 8.8kb insert. However, I cannot get any amplified product at all. I have tried changing some parameters but I am not sure what to do. Any help?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing PCR Hot start PCR - Mammalian DNA using Hot Start Taq DNA Polymerase from GenScript.

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Manufacturer protocol

Download the product protocol from GenScript for Hot Start Taq DNA Polymerase below.

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Videos

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