EpiTect MSP Kit

PCR Methylation specific PCR - Mammalian DNA

Experiment
PCR Methylation specific PCR - Mammalian DNA
Product
EpiTect MSP Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.

Publication protocol

MSP
MSP was carried out in a total volume of 50 μl (EpiTect Master Mix, 25 μl), with modified DNA (4 μl) in RNase-free water. The reaction conditions for MSP were: initial denaturation at 95°C for 10 min, followed by 94°C for 30 sec; 58°C for 45 sec; 72°C for 45 sec for 40 cycles; and 72°C for 10 min for both methylated and unmethylated reactions. The PCR products (6 μl) were electrophoresed, verified in 1.5% agarose gel and observed under UV light. Target gene amplification of methylation primers was considered fully methylated, while that of unmethylated primers was considered partially methylated.

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Reviews

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Discussion

Discussion

5 years ago

Author: United Kingdom

How “strong” is the PCR product of methylation specific PCR?

Does anyone know how “strong” the PCR product of methylation specific PCR is? I kept my PCR products at 4C for about 3 weeks and then at room temperature for another week. Will I be able to use them for sequencing?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing PCR Methylation specific PCR - Mammalian DNA using EpiTect MSP Kit from Qiagen.

Paper title
Methylation status of WWOX gene promoter CpG islands in epithelial ovarian cancer and its clinical significance
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Manufacturer protocol

Download the product protocol from Qiagen for EpiTect MSP Kit below.

Download PDF Download manufacturer protocol

Videos

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