DreamTaq DNA Polymerases

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp

Publication protocol

Conventional and real-time PCR amplifications
Amplification reactions with the two selected primer pairs, one specific for the strains of 1 and the second specific for 2, were performed in a Biometra T3000 thermocycler (Biometra, Göttingen, Germany). The reaction mixture in 15 μl of total reaction volume contained 10 ng of DNA, 0.4 U of Dream DNA Polymerase (Promega, Madison, WI, USA), 1× reaction Dream Taq Green buffer (Thermo Scientific, Vilnius, Lithuania), 0.15 mM dNTPs and 0.7 mM of each primer. The following experimentally determined amplification conditions were used: initial denaturation at 94 °C for 4 min; 30 cycles at 94 °C for 45 s, 55–62 °C for 45 s for primers Psm1-6F and Psm1-6R (for detection of 1 strains) and 50–58 °C for 45 s for primers Psm2-8F and Psm2-8R (for detection of 2 strains) and 72 °C for 1 min; and final extension at 72 °C for 10 min. The resulting PCR products were separated by electrophoresis on 1.5 % agarose gels as described above.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DreamTaq DNA Polymerases below.

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