QuantiFast Multiplex PCR Kit

PCR Multiplex PCR - Bacterial DNA

Experiment
PCR Multiplex PCR - Bacterial DNA
Product
QuantiFast Multiplex PCR Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.
Protocol tips
PCR plates, tubes and tips should be UV sterilized for 20-30 mins

Publication protocol

Reactions were carried out on the ABI 7500 Fast instrument (Applied Biosystems, Paisley, UK). mRT-PCR assays were carried out in a total volume of 20 μL, comprising 10 μL of QuantiFast Multiplex PCR mastermix (Qiagen), 2 μL of nuclease-free water (Promega), 2 μL of oligonucleotide mixture (), and 6 μL of nucleic acid extract. Cycle parameters were 95°C for 5 min, followed by 45 cycles of 95°C for 45 s and 60°C for 45 s. real-time PCR was carried out with the same reaction components, but with different cycle parameters: 95°C for 5 min, followed by 45 cycles of 95°C for 30 s and 60°C for 30 s.

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Discussion

Discussion

5 years ago

Author: R. Verma India

Multiplex PCR and qPCR

I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?

Discussion

5 years ago

Author: Roland Lafontaine France

My multiplex PCR has decreased in sensitivity

Hello there! My multiplex PCR has decreased in sensitivity even though I have not changed any of the parameters. The materials are in good condition and the PCR machine is well calibrated. I have tested multiple samples but one of my targets has decreased in sensitivity.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing PCR Multiplex PCR - Bacterial DNA using QuantiFast Multiplex PCR Kit from Qiagen.

Paper title
Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections
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Manufacturer protocol

Download the product protocol from Qiagen for QuantiFast Multiplex PCR Kit below.

Download PDF Download manufacturer protocol

Videos

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