Promega PCR Master Mix

PCR Multiplex PCR - Bacterial DNA

Experiment
PCR Multiplex PCR - Bacterial DNA
Product
Promega PCR Master Mix from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp
Protocol tips
PCR plates, tubes and tips should be UV sterilized for 20-30 mins

Publication protocol

DNA extracts (1 μL) from all mycobacterial reference strains and clinical isolates, were amplified by multiplex-PCR using 24 μL reaction mixture containing 0.2 pmol/μL TB1 and TB2 primers (Invitrogen - Integrated DNA Technologies, Inc. Coralville, USA), 0.4 pmol/μL pncATB-1.2 and pncAMB-2 primers (Invitrogen - Integrated DNA Technologies, Inc. Coralville, USA) and PCR Master Mix (Promega Corporation, Madison, Wisconsin, USA), according to manufacturer’s instructions.

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Reviews

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Discussion

Discussion

5 years ago

Author: R. Verma India

Multiplex PCR and qPCR

I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?

Discussion

5 years ago

Author: Roland Lafontaine France

My multiplex PCR has decreased in sensitivity

Hello there! My multiplex PCR has decreased in sensitivity even though I have not changed any of the parameters. The materials are in good condition and the PCR machine is well calibrated. I have tested multiple samples but one of my targets has decreased in sensitivity.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing PCR Multiplex PCR - Bacterial DNA using Promega PCR Master Mix from Promega.

Paper title
Multiplex-PCR for differentiation of from complex
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Manufacturer protocol

Download the product protocol from Promega for Promega PCR Master Mix below.

Download PDF Download manufacturer protocol

Videos

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