SYBR GreenER™ qPCR SuperMix Universal

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.	PCR plates, tubes and tips should be UV sterilized for 20-30 mins

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.
Protocol tips
PCR plates, tubes and tips should be UV sterilized for 20-30 mins

Publication protocol

The reaction mix contained 2 µL of extracted DNA, 10 pM each primer, 1µL of BSA, 10 µL of SYBR green qPCR Supermix (Invitrogen) for a final volume of 20 µL. The multiplex qPCR was performed using the LightCycler®1.5 Real-Time PCR System (Roche Diagnostics) under the following conditions: 96 °C for two min (DNA denaturation), followed by 40 cycles of 96 °C for two sec, 60 °C for five sec and 72 °C for eight sec. A melting curve analysis was performed at the end of the amplification cycles. Positive reactions were recognized by typical melting temperatures (Tm ∼ 82 °C).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for SYBR GreenER™ qPCR SuperMix Universal below.

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