Platinum™ Taq DNA Polymerase

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.	Always thaw on ice

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.
Protocol tips
Always thaw on ice

Publication protocol

After thawing at room temperature, the samples
were subjected to the process of DNA extraction with
the DNeasy Blood & Tissue Kit (QIAGEN, Germany).
Then, a PCR was carried out to check the efficiency
of extraction of DNA using the primer pair ISO2G14.
DNA amplification was carried out in a final volume
of 25 µL, containing 5 U of Platinum Taq DNA
Polymerase, 5 pmol of primers pair, 5 µL of reaction
buffer 5X, 25 mM of MgCl2, 10 mM of dNTP, 2 µL
of sample and water. The PCR cycling protocol was:
95° C for 2 min, 40 cycles of 95° C for 1 min, 50° C
for 1 min and 72° C for 1 min, elongation at 72° C for
5 min

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Platinum™ Taq DNA Polymerase below.

Download manufacturer protocol

Videos

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