anti-p62 Protein, C-Terminal Specific Polyclonal Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Lyse cells in in RIPA lysis buffer containing protease inhibitors	Dilute primary Ab at 1:1,000 for immunoblotting

Upstream tips
Lyse cells in in RIPA lysis buffer containing protease inhibitors
Protocol tips
Dilute primary Ab at 1:1,000 for immunoblotting

Publication protocol

Cells were cultured in normal culture medium under normoxia (21% O2) or hypoxia (1% O2) conditions for the indicated times. Cells were subsequently lysed in RIPA lysis buffer containing protease inhibitors and subjected to immunoblotting with primary antibodies: Flag (sigma, cat#F1804_200UG); p62 (American Research Products, cat#03-GP62-C); HIF1 (Cell Signaling, cat#3716S); β-actin (Sigma-Aldrich, cat#A5441); mCherry (Abcam, cat#ab125096); Fibronectin (Abcam, cat#ab2413); Tom20 (Santa Cruz, cat#sc-11415); LOX (abcam, cat#ab174316); HSP60 (Cell signaling, cat#12165P) and detection with a Li-Cor Odyssey CLx Imager.

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Manufacturer protocol

Download the product protocol from ARP American Research Products for anti-p62 Protein, C-Terminal Specific Polyclonal Antibody below.

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