Autophagy Assay Kit

Protocol tips

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	Add detection reagent and incubate the cells at 37 °C with 5% CO2 for 15 minutes to 1 hour

Protocol tips
Add detection reagent and incubate the cells at 37 °C with 5% CO2 for 15 minutes to 1 hour

Publication protocol

Cardiomyocytes were isolated from hearts and incubated with serum-free MEM. Cells were washed with PBS and incubated with detection reagent from an autophagosome detection kit (MAK138; Sigma-Aldrich), sarcomeric alpha actinin antibody (ab9465) and 4',6-diamidino-2-phenylindole for 15 min. Following three washes, fluorescence intensity was measured at Ex/Em (360/520 nm) using a microplate reader (BioTek-SYNERGY H1) and Zeiss LSM 510 confocal microscope.

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