LC3B Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Dilute primary Ab at 1:1000

Protocol tips
- Dilute primary Ab at 1:1000

Publication protocol

Western blot analysis was carried out following standard methods. Cells were lysed with RIPA lysis buffer (Cell Signalling Technologies Inc, 9806, USA). Protein detection was performed using Amersham Imager 600 (GE Healthcare Life Sciences) chemiluminescence (Invitrogen, USA). Band intensities were quantified by using the software ImageJ (NIH, USA). LC3 conversion was quantified by measuring band intensities of LC3-II and normalized to β-actin.

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Manufacturer protocol

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Videos

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