Anti-Atg5

Protocol tips

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	Use 2-5 μg/mL for WB

Protocol tips
Use 2-5 μg/mL for WB

Publication protocol

Western blotting with Immunostar® LD chemiluminescent substrates (Wako, 290–69904) was performed. Signals were detected with an LAS-3000 image analyzer (Fujifilm, Tokyo, Japan) and analyzed using Multigauge software (Fujifilm) as described previously.20,55,56 LC3 (PM036), SQSTM1 (PM045), ATG5 (M153–3), BECN1 (PD017) and LMNB1/Lamin B1 (PM064) were purchased from MBL. LAMP2 (ab13524), CTSB (ab58802), CTSL (ab133641), TFEB (ab2636) and CIDEC (ab16760) were purchased from Abcam. CDKN1A (sc-397) and CASP1 (sc-514) were purchased from Santa Cruz Biotechnology. FLAG M2 (F1804) and ACTB/β-actin (A1978) were purchased from Sigma-Aldrich. TP53 (OP03) was purchased from Millipore. GAPDH (010–25521) was purchased from WAKO.

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Manufacturer protocol

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