LC3B (D11) XP® Rabbit mAb

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Incubate Primary Ab membrane overnight at 4 °C with constant shaking

Protocol tips
- Incubate Primary Ab membrane overnight at 4 °C with constant shaking

Publication protocol

Cells were harvested and lysed with RIPA buffer (Cell Signaling Technologies, Beverly, MA, USA) after treatments. Protein concentrations were quantitated by using Bradford reagent (Bio-Rad, Hercules, CA, USA) before electrophoresis. After electrophoresis, proteins were transferred to the membrane, which was then blocked with 5% non-fat dried milk. Corresponding primary antibodies were incubated with the membrane overnight at 4 °C with constant shaking. The membrane was then incubated with HRP-conjugated secondary antibodies before being visualized by using the ECL Western Blotting Detection Reagents (Invitrogen, Paisley, Scotland). Band intensities were quantified by using the software, ImageJ (ImageJ 1.46r; National Institutes of Health, Bethesda, MD, USA).

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for LC3B (D11) XP® Rabbit mAb below.

Download manufacturer protocol

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